ABSTRACT
High performance thin layer chromatographic method for quantitative estimation of esculin and capsaicin in some phytopharmaceuticals was presented. Acid hydrolysis of esculin and alkaline hydrolysis of capsaicin were carried out for the preparation of their degradation products. The degradates were isolated, identified and confirmed by UV, Mass and IR spectroscopy. The proposed method based on the determination of esculin in presence of its degradates at 365 nm using mobile phase; butanol: glacial acetic acid: water [5:1:4:v/v/v], linearity range is 2.0 - 10.0 micro g/spot. Capsaicin was determined in presence of its degradates at 254 nm using mobile phase; toluene: diethyl ether: dioxane, [62.5: 21.5: 16 v/v/v], linearity range is 4.0 - 16.0 microg/spot. The suggested method was applied for the determination of esculin and capsaicin in pure form, and different dose forms as stability indicating method. The validity was assessed by applying the standard addition technique, and all validation parameters were performed according to ICH guidelines. Statistical analysis was carried out recording an accurate, precise, and reproducible results compared to the published methods